FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109166
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCG3650.0145.000025
CCGGGTT2200.0145.000029
ATGGCCG2150.0145.000025
CCGGACT1100.0145.000029
GCGCCCG2900.0145.000025
TGGCCGG8600.0145.000026
AGTGCCG2150.0145.000025
AAGCCCG2900.0145.000025
CGAGCCG2150.0145.000025
GGCTGCC550.0145.000024
GTGCCGG8800.0145.000026
TCACGGC253.711648E-8145.03
TCACGGA151.2300414E-4145.01
CCCTGAA253.711648E-8145.02
TCACGAT151.2300414E-4145.01
TCTGCCG4250.0145.05
TGTTTTG151.2300414E-4145.01
TTTAGTC450.0145.03
GCCCCGG16150.0145.06
CTAGATT151.2300414E-4145.02
Produced by FastQC (version 0.11.2)