FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705648
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATGG4250.0145.000025
AACTTGG3300.0145.000025
AGTGGAA27500.0145.000027
TTGGTGG1650.0145.000025
TGAATGG1650.0145.000025
GACCTGG3300.0145.000025
TTCCTGG5100.0145.000025
ACGGTGG1650.0145.000025
CTTGTTC151.229853E-4145.02
CTTGTTA151.229853E-4145.01
TAATCAC100.0070904233145.02
TCACGGT351.0913936E-11145.03
TGATCGC100.0070904233145.01
ACGCGAT100.0070904233145.03
GCGAGAT100.0070904233145.03
CCACAAG202.1301676E-6145.02
TCAAAAA100.0070904233145.01
TCACGGA100.0070904233145.02
GCGAGAA100.0070904233145.02
CGGACAA100.0070904233145.02
Produced by FastQC (version 0.11.2)