FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100778
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTG100.007081469145.0145
CGACAGC100.007081469145.01
TTGTCTT253.6869096E-8145.04
AACGCTC100.007081469145.02
ACAGCAT100.007081469145.03
CTCTGAT151.2267502E-4145.03
ATCCTAT100.007081469145.03
ACAGTAT100.007081469145.03
TCCTTTA100.007081469145.02
TTTGACT151.2267502E-4145.03
AAACCTT151.2267502E-4145.04
TTCACTT253.6869096E-8145.04
CAATGTG100.007081469145.01
GAGGCAC100.007081469145.02
GAGCGTT100.007081469145.04
ACTGTGT100.007081469145.03
ACTGCCC100.007081469145.02
TTGCATT253.6869096E-8145.04
CATGATA100.007081469145.01
AACAATA100.007081469145.02
Produced by FastQC (version 0.11.2)