FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177867
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTCC100.0070859953145.000023
GCCCCAC100.0070859953145.000023
ATGGGTC100.0070859953145.000022
CAGCCAT800.0145.000025
GCCCTAC100.0070859953145.000023
CAGCCAC100.0070859953145.000023
CGACAGT100.0070859953145.000022
CTTTCCT100.0070859953145.000022
CAGCTAG100.0070859953145.000022
CTCGAAT100.0070859953145.000021
CAGCTAA100.0070859953145.000022
CGTTTCT100.0070859953145.000021
TGGTCTC100.0070859953145.000023
CGTTTCC100.0070859953145.000023
ATCCCCA100.0070859953145.000024
CTTTTAC100.0070859953145.000023
GTATGTC100.0070859953145.000023
CGTTTAC100.0070859953145.000021
GGATGTC100.0070859953145.000023
ACAGTCA202.125742E-6145.000024
Produced by FastQC (version 0.11.2)