FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534201
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC100.0070912284145.01
TCGCCGC100.0070912284145.01
GTTGAGT100.0070912284145.01
CAGGTTC100.0070912284145.01
TTGGCCG100.0070912284145.01
TTGGCCC151.2301326E-4145.01
ACCGGTT151.2301326E-4145.02
ACCGGGC202.1309734E-6145.02
GCAAGGC100.0070912284145.02
TGCCCCG253.7123755E-8145.02
GTTCTGG151.2301326E-4145.01
GAGCTTG151.2301326E-4145.01
TCCGCGT202.1309734E-6145.01
GATCGTG151.2301326E-4145.02
GGTAATC100.0070912284145.01
GGGCTTG151.2301326E-4145.01
CTCCGGG100.0070912284145.01
GCTGGTG100.0070912284145.01
GGGTCTT100.0070912284145.01
GCGCGGA100.0070912284145.01
Produced by FastQC (version 0.11.2)