FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859899
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACAA3650.0145.000025
GCGTCAA2050.0145.000025
GCATAAG100.0070906905145.01
GGGGCAT100.0070906905145.01
GGGGCAA1250.0145.05
CGGACAC151.2299458E-4145.03
GCGAGAA100.0070906905145.08
CCCTGCG100.0070906905145.02
CCCTGAT253.7111022E-8145.01
CCCTGAG100.0070906905145.01
TCACGCT100.0070906905145.01
TCACGAT100.0070906905145.02
CCTTCGC202.130435E-6145.03
ATTTATT202.130435E-6145.02
TATTTTG151.2299458E-4145.01
CTTGTGG100.0070906905145.01
GCCCCTT151.2299458E-4145.01
GCCCCTG253.7111022E-8145.02
TCTGCAG100.0070906905145.02
GTCATTG100.0070906905145.01
Produced by FastQC (version 0.11.2)