FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835502
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGC1100.0145.000024
TGTGGGC550.0145.000024
TCTGCGG253.7127393E-8145.03
ATGGGTG306.511982E-10145.03
CATGGGT202.1310861E-6145.02
CTAGACC100.0070913415145.01
ATGGGCC100.0070913415145.01
TGGAGTC151.2301716E-4145.02
CGTGGCG253.7127393E-8145.03
GAGTGGC500.0145.04
GGGGAGC306.511982E-10145.04
GGGGACG100.0070913415145.03
GACAGCC100.0070913415145.01
GGCAGGT151.2301716E-4145.01
TGGAGAC151.2301716E-4145.02
CGGCGAC151.2301716E-4145.01
GTCGCAG306.511982E-10145.03
GGAGCTC202.1310861E-6145.02
TCCGCAC351.0913936E-11145.01
GTCTGTA100.0070913415145.01
Produced by FastQC (version 0.11.2)