FastQCFastQC Report
Mon 23 Oct 2017
000000000-BH5K9_l01n02.332000000a0c59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02.332000000a0c59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13939585
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTTT926750.0141.862969
AGGCGTC405000.0141.706188
GATCTGC1355250.0141.667229
CACCGAC526000.0141.278529
GCACTCT782650.0141.072339
GGCGTCC1671000.0141.060449
GGGCGTC292350.0140.36268
CCTGCAC1118100.0140.305459
TGTTTGA1241800.0140.265149
CAGGCGT137450.0139.989097
CCACCGA302250.0139.650978
CGATCTG439700.0138.800318
GCTTAAG334150.0138.729619
TCCTGCA380850.0138.470548
ATCGGAT9387900.0138.44266145
CTTCTCA2075550.0138.282879
CGCACTC253400.0138.19068
GGCACTC142200.0138.066128
TGCACTC210700.0137.739698
TGGGCGT88200.0137.108847
Produced by FastQC (version 0.11.2)