FastQCFastQC Report
Thu 16 Nov 2017
000000000-BH4HJ_l01n02.332000000a8860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH4HJ_l01n02.332000000a8860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20016415
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGGAGATAG6002092.9985839122540177No Hit
GGCCACGTAGTA5620602.807995337826479No Hit
GAACTAGTCACC5131972.5638806949196447No Hit
CCAGTGTATGCA4979362.4876382708891676No Hit
TCTAGCGTAGTG4890982.443484510088345No Hit
GTCAATTGACCG4861902.428956434006789No Hit
GGAGACAAGGGA4856562.4262886236121703No Hit
TCCAAAGTGTTC4843832.419928843401778No Hit
CCTGAACTAGTT4701172.3486573394886148No Hit
CAAGCATGCCTA4694972.3455598817270724No Hit
TCCCAGAACAAC4694702.345424992437457No Hit
TAGGAACTGGCC4484562.2404411579196375No Hit
CGGAGCTATGGT4448182.222266075118846No Hit
GACTTTCCCTCG4377612.1870100115330344No Hit
ATGAGACTCCAC4298172.1473225849883706No Hit
TTACTGTGCGAT4233592.115059065272178No Hit
GCGATATATCGC4204962.1007558046733146No Hit
TACACGATCTAC4092412.0445269545020923No Hit
ATCCCGAATTTG4031622.0141568807401327No Hit
AATCCGTACAGC4026612.0116539350328217No Hit
TAGGATTGCTCG3979471.98810326424587No Hit
AGCTGGAAGTCC3977211.9869741909327918No Hit
ACGCGCAGATAC3976621.986679432855484No Hit
ATGTGCACGACT3916961.9568738957500633No Hit
TATCGTTGACCA3837441.9171465020084766No Hit
GACGGAACCCAT3815621.9062454490476939No Hit
AGGCATCTTACG3737911.8674223131364933No Hit
CTAGCGAACATC3641921.8194666727283582No Hit
ACACGTAAGCCT3403771.7004893233878295No Hit
ATTCCTGTGAGT3272911.6351129810208271No Hit
AAGAGATGTCGA3229281.6133158709988777No Hit
GAATCTTCGAGC2914861.4562347952917643No Hit
CCTCGTTCGACT2895661.4466426680302142No Hit
AATCAGTCTCGT2820901.4092933225055535No Hit
TCGAGGACTGCA2689291.343542287667397No Hit
TGAGTCACTGGT2572631.2852601227542495No Hit
CAGCTAGAACGC2433961.215981982787627No Hit
GAGTGGTAGAGA2321741.159917997303713No Hit
CTTCGGCAGAAT1097930.5485148064725877No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGA550.0091592356.00011251
GACAGTC1001.2863165E-66.00011251
AATCCGG1001.2863165E-66.00011251
GACCGGT806.538044E-56.00011251
GACGGAC1303.6452548E-96.00011251
ATGCGGC1054.8296715E-76.00011251
TCGAGGC1601.0913936E-116.00011251
TACAGTT650.0012617446.00011251
TACAGGC704.6965055E-46.00011251
CCGGGGT852.4444593E-56.0001121
GGCCACA2850.06.0001121
CGTCTGA806.5401764E-55.9999784
GCAGATG806.5401764E-55.9999785
GTGGTAC806.5401764E-55.9999783
TCACGGG751.7515583E-45.99997765
GTTTGTG550.00916136155.99997764
GTTGACG1054.831709E-75.99997765
ACACGGG6500.05.99997765
CGTTGCT1156.828668E-85.99997764
TGTGGAC1653.6379788E-125.99997762
Produced by FastQC (version 0.11.2)