FastQCFastQC Report
Wed 2 May 2018
000000000-BH4B9_l01n01.331000000d31fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH4B9_l01n01.331000000d31fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14420540
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC312950.21701683848177666RNA PCR Primer, Index 4 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC69200.036.8009544
GTATGCC69950.036.27529545
TCGTATG75000.033.67994343
CTCGTAT64800.032.54347242
TCTCGTA75850.027.22421541
ATCTCGT88800.023.14685440
CAATCTC90100.023.04501538
GGCTACA36800.022.16535424
ACTGCCT50700.022.1292257
CCAATCT94650.021.93719937
AATCTCG97100.021.86584339
ACCAATC97400.021.31782336
CACTGCC52250.021.2707616
AATCCAC57900.019.233432
GCTACAT43350.018.81626325
GACCAAT135600.018.51269135
TGGCTAC45250.018.35975523
CCTTGAT60800.018.1336811
TACATCC45050.018.05232827
CTGCCTT67250.017.8019088
Produced by FastQC (version 0.11.2)