FastQCFastQC Report
Fri 9 Mar 2018
000000000-BH38T_l01n03.333000000c9f55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH38T_l01n03.333000000c9f55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18744852
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG11804996.297723769704877No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG10957915.845823696020646No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA5490652.9291508943362157No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA4466362.3827128643106916No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA4302892.2955049204976383No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGGA4164592.221724663390247No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3542521.8898628807525395No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA3235781.7262232851985175No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3117291.6630112630390468No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCTCTGTCTAGGT2689921.4350179985416796No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA2237741.193789100068648No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA2007371.070891357264384No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT1944121.0371487595634257No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1920531.0245639709505308No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA1867490.9962682020642254No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1798720.9595807958366382No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1730440.9231547947137699No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1694880.9041842528284565No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG1689170.9011380831387732No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1666510.8890494307450387No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG1590040.8482542300147262No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTGATATCCAGAA1421900.7585549355097602No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG1324630.7066633548240338No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG1293180.6898854149395258No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACACTTTAGCA1214280.6477938582817299No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG1182700.6309465660225005No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTG1073820.5728612847943532No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1066150.5687694946857943No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTG909960.48544528385713576No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA908470.4846503989468682No Hit
CCTGTTTGCTCCCCTTGCTTTCGCGCCTTAGCGTCAGGTGTAAATTAGAA903550.48202567830356835No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTACGGTCCAGTA901260.48080400954886177No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG900020.48014249458998126No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG880930.4699583651020557No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG875940.4672963008723675No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG875660.46714692652681383No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG832960.4443673388298825No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGACCAGAC827330.44136384752464297No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTACAGGCCAGGC825710.44049961023965406No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTACAGTCCAGTG817800.43627978497776354No Hit
CCTGTTCGCTCCCCATGCTCTCGCTCCTCAGCGTCAGTTACTGCCCAGAG801250.4274506941959318No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA785460.4190270480663171No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC752170.4012675053395994No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA733860.39149949010000185No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG699710.3732811547405122No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG650110.34682055638529447No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA639590.34120834883092166No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG611640.32629758826583427No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAA598450.31926098963064636No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA586950.3131259718668357No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA577680.3081806140693989No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGCCCAGGA566570.30225365342975236No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA539690.2879137162566021No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA522640.278817885571996No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCTTCAGTGTCAGTATTCGTCCAGTG505630.26974339407961184No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTATCAGTCCAGTA485520.25901511519002657No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAATGTCAGTTTCTGTCCAGAA437870.23359480245562889No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA435550.2323571293067558No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTCACTGCCCAGTA425690.22709701842404517No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGCAGACCAGGA422180.22522450430656907No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA409650.2185400023430433No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTG405770.21647010069751416No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA399190.21295980357700345No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTAACGGCCCAGAG352290.1879396006967673No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTTTGTGTCCAGCA344940.18401852412598402No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA344340.18369843624265478No Hit
CCTGTTCGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA337050.17980936846020443No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA325080.17342361518778596No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTAAAGACCAGAA304190.1622792220498727No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAG296830.15835281068103393No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA290340.15489052674302256No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT285500.15230848448416662No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA270870.1445036749289885No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG259850.13862472747184135No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTGCTGTCCAGAA257430.13733370634241338No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG251740.13429820624884103No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTATCGGCCCAGAG249560.13313522027274474No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG249360.133028524311635No Hit
CCTGTTCGCTACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG244450.13040913846639066No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTTAAAGTCCAGCA225270.12017699579596575No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCCGTCCAGAA224610.11982489912430357No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA223740.11936077169347616No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA214250.11429804833881857No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA213620.11396195606132287No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA211930.11306037518994548No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAGGA210590.11234551225051018No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTAACGGCCCAGTA208760.11136924420635597No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG200440.10693069222419041No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAA198140.10570368867142829No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGA193910.1034470690939571No Hit
CCCGTTCGCTACCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA193450.1032016683834047No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTTGATGTCCAGTA192500.10269486256813337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCTC7944750.0144.612885
TGTTTGC8878500.0144.601033
TTGATAC1684250.0144.564226
TTGCTCC7976200.0144.538076
CTGTTCG4752250.0144.50742
TGTTTGA1817500.0144.493533
ATTTGCT1076550.0144.47154
CTATTTG1081900.0144.439392
TGTTCGA1592800.0144.435973
CTGTTTG10703900.0144.359072
TGCTCCC7986100.0144.323557
GTTTGCT9197200.0144.3214
CCTGTTC4743700.0144.200351
ATACCCG1968700.0144.14129
TTTTGAA943550.0144.13448145
TGTTCGC3174350.0143.999473
GATACCC3250750.0143.798698
TTTCAAA440600.0143.72203145
CCTGTTT10730000.0143.706451
GTTCGAT1601850.0143.701284
Produced by FastQC (version 0.11.2)