FastQCFastQC Report
Fri 9 Mar 2018
000000000-BH38T_l01n02.332000000c9f58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH38T_l01n02.332000000c9f58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18744852
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAAGGTAA5715873.0493012161419037No Hit
TAGTTAACGTCC4671242.4920122068715185No Hit
ACAGACCACTCA4405902.3504586752672147No Hit
TCCAATAGCGTG4023122.1462532752992662No Hit
GTGTGGCCAAAC3827702.04200065169893No Hit
TCGCAAACTTTG3800232.0273459614405067No Hit
AGAGAAATGTCG3691301.969234006222082No Hit
GTGTTTGGTCGA3689991.968535147676813No Hit
GCGCGAACAGTT3625691.9342323961800287No Hit
TGGGCAACCTGA3563911.901274013793227No Hit
ACGTCTGTAGCA3554501.8962539688230133No Hit
CCTTGTGCCTTG3476171.8544664956543802No Hit
ACGCAACTGCTA3471151.8517884270305256No Hit
TGAGTGGAATGA3402201.8150049944379396No Hit
GCATGAAATGGA3399351.8134845769921255No Hit
AGAAGTCCCTGG3376491.8012892286372812No Hit
GGCCATTAGTCA3326401.7745672251773446No Hit
CAGCACTAAGCG3307521.7644951264485846No Hit
GGTGCGTGTATG3248731.7331318486803737No Hit
TCGTGATGTGAC3244701.7309819250640124Illumina PCR Primer Index 1 (100% over 12bp)
GCGTTACACACA3177811.6952974608708566No Hit
CAAGTGAGAGAG3163901.6878767567756736No Hit
CTACGACCATTA3151211.6811068980432602No Hit
GTTGCTTGCAAT3150781.680877501726874No Hit
AGATCGGCTCGA3146911.6788129348794008No Hit
TAGTGCTGCGTA3129601.669578399445352No Hit
GTTACTGGGTGG3055371.6299781934794686No Hit
TCCACAGGAGTT3047041.6255343066992474No Hit
TCATCGCGATAT3026051.6143365655807793No Hit
GGAAGTGAAGTT3000941.60094088766345No Hit
GACTTATAGGCC2966871.5827652306884044No Hit
TATATCCACTGG2920281.5579104065478884No Hit
GAACATGATGAG2887561.5404549473103335No Hit
ACATGTCACGTG2860271.525896283416908No Hit
CTACATCTAAGC2843701.5170565230389657No Hit
CTTGACTGAGGT2832911.5113002759370946No Hit
GTAGATGCTTCG2773001.4793395007866692No Hit
AAGTTCGCGCTA2694531.4374773404452594No Hit
TAGTCAGGCCAT2649281.4133373792441788No Hit
GAAGGAAGCAGG2594561.3841453642845514No Hit
TCGACGGTGCAA2558841.36508946563035No Hit
TTCGTGCCTGCT2411451.2864598770905205No Hit
AGATTACCGGCG2338211.2473878161321306No Hit
GATGATCGCCGA2088311.1140712127254992No Hit
ACGAGTGCTATC2036351.0863516020291863No Hit
TTGCACGATTGG1933971.0317339395371061No Hit
TCAAGGCATCGG1903201.015318765920371No Hit
GCTAAGAGAGTA1816730.9691887671345711No Hit
TTCGCGTGAAAC1569190.8371311760690349No Hit
CAAGGTTCTTGT1515240.80834994055968No Hit
CCGCTACGTCTT493540.2632936232305275No Hit
CGCAAGGATTGC463430.2472305462854548No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGTT650.00126201356.00000051
CTCGAAC1303.6470738E-96.00000052
GTCCCAA650.00126201356.00000055
CTAGGAC650.00126201356.00000051
GAAGCCC1552.7284841E-116.02
GAAGCAT751.7514436E-46.05
CGAACGT806.5397166E-56.04
ACCGGCC953.4300883E-66.06
CGAGTCC550.0091609376.02
CACGGGA953.4300883E-66.03
GACACGT909.153015E-66.05
CCCTAAG550.0091609376.04
AGCCCGC600.00339666616.03
TAGGTTC852.4451161E-56.02
GGCGTAT751.7514436E-46.01
CGCATAC2550.06.02
CGTCTGG2500.06.02
CACGAAC909.153015E-66.02
GGGAGCA751.7514436E-46.04
CGCTGCA600.00339666616.05
Produced by FastQC (version 0.11.2)