FastQCFastQC Report
Fri 11 May 2018
000000000-BGWPT_l01n03.333000000db068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BGWPT_l01n03.333000000db068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10393569
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG212017020.398863951352993No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAA8587238.26206089554031No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG5927555.703093903547472No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG5427765.222229245796126No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG4226754.066697397207831No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA2950632.8388997080791016No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG2751902.6476949352046444No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT2383002.2927639196891847No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2005381.9294431008251351No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1696611.6323651673453077No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG1487421.4310964789861884No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1171791.127418310303227No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1134861.0918867234152194No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCCTCAGCGTCAGTTATAGACCAGAA1039861.0004840493193434No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA828710.7973295794736148No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA748630.720281935877849No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGAA717510.6903403441108632No Hit
CCTATTTGCTCCCCACACTTTCGAGCCTAAGCGTCAGTGATAGTCCAAGT528180.50817962530484No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTTGGCCCAGGT465060.44744976436871686No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGTGTCAATATACAACCAGTT454280.4370779661923637No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG446750.4298331016035012No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTATTATCCCAGGG392080.37723326799485335No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA381070.3666401791338471No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAATTTGTTAGTA377510.36321498418878057No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG360300.346656668176254No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG353150.3397774142837749No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACACTCCCAAGG327420.31502172160496555No Hit
CCTGTTCGCTCCCCACGCTTTCGTGACTCAGCGTCAGTTTCAGTCCAGTA321380.30921043579929086No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG291080.2800577934297641No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG289210.27825860395019264No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG238820.22977670134291697No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGTGTCAGTGCCGGTCCAGTG212030.2040011472478799No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTGCAGACCAGAA155620.14972720150316027No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATTGGCCCAGGG153180.14737959597901357No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAATTACAGACCAGAG148960.1433193929823336No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATAACTTGGTA140100.13479489095612876No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG137920.13269744011898127No Hit
CGTTTTTGATCCCCTAACCTTCGTTCTTGATCAATGAAAACGTCCTTGGC126540.12174836189570687No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCATGAGCGTCAGTATTGGCCCAGGG112480.1082207661295172No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG109860.1056999765912941No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA105230.10124529889588456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCTC6611400.0244.89815
TGTTTGC6659600.0244.856523
CTGTTCG836800.0244.575472
TGTTTGA566150.0244.480713
CCTGTTC839650.0244.022521
TTTGATC536800.0243.607965
CTCCCCA7235900.0243.34949
GTTTGCT6704350.0243.28614
TGATCCC543050.0242.428447
CTGTTTG7292050.0242.417972
GCTCCCC7387050.0241.908928
TTGCTCC6740400.0241.688696
CTATTTG70200.0241.684482
ATTCGCT90500.0241.616034
CCATTCG89350.0241.161162
TGCTCCC6794200.0240.332037
CCCTCCG1488400.0239.37048245
TTGATCC551050.0238.575456
CCTGTTT7410700.0238.46731
TGTTCGC859900.0237.478213
Produced by FastQC (version 0.11.2)