FastQCFastQC Report
Fri 11 May 2018
000000000-BGWPT_l01n02.332000000db06b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BGWPT_l01n02.332000000db06b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10393569
Sequences flagged as poor quality0
Sequence length12
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGATTTGCCA180992417.413883527400454No Hit
ATTAGTTCGCGT145730614.021227934312073No Hit
ATACTTCGCAGG10384769.991524566777784No Hit
CCATGCGATAAC3425043.2953454198456757No Hit
TAACGCTTGGGT3018252.9039591693671345No Hit
TGTAATTGTCGC2432902.340774376924808No Hit
ATTGGGCTAGGC2423682.33190350687045No Hit
AGCCTAAGCACG1652311.5897426572142832No Hit
ATGTCGAGAGAA1418491.364776622929044No Hit
CCTCTCGTGATC1317371.2674856923545703No Hit
AACACAAGGAGT1282951.2343690603295172No Hit
AGCTTGACAGCT1129001.0862486216236211No Hit
AATCTTGCTGCA1106261.0643697078453032No Hit
ACTCACGGTATG900750.8666416704406349No Hit
CAACACGCACGA879910.8465908101442343No Hit
GTCCGAAACACT720550.6932652296819313No Hit
GTACCTAATTGC656430.6315732353342726No Hit
TCTTCCGCTACT609540.5864587996673712No Hit
ATCGCTCGAGGA588140.5658691446605107No Hit
ACCACATACATC519770.500088083313826No Hit
TCAACAGCATCG460610.44316827068738374No Hit
CTCTACCTCTAC420010.4041056541790409No Hit
TCGTCGATAATC391190.3763769692585867No Hit
GCAGGATAGATA374770.36057873864117324No Hit
CACATCTAACAC368550.35459426882142214No Hit
TTATGCAGTCGT360750.34708962821144496No Hit
TGCAATGTTGCT355540.34207691313734484No Hit
CCATAGGGTTCA347490.3343317391744838No Hit
GCCTGAATTTAC343070.3300791094954967No Hit
TATCAGGTGTGC333970.3213236954505233No Hit
GACACATTTCTG306290.29469184261921966No Hit
ATGATGACCCGT301790.2903622422673097No Hit
GTATTACGATCC299070.28774523938793306No Hit
TGTTATCGCACA297550.286282796602399No Hit
CGCCAAATAACC296560.28533028452497883No Hit
CGTGACAATGTC295030.2838582204053295No Hit
TCTGTTGCTCTC290990.2799712014227259No Hit
TAAACCGCGTGT281020.2703787313097166No Hit
TAACACCACATC279100.2685314351595684No Hit
TACTTCGCTCGC268300.2581403943149846No Hit
TGGCAAGACTCT267070.2569569702187959No Hit
TCGGAGTGTTGT264490.2544746660170342No Hit
AACGAGAACTGA260680.2508089377190838No Hit
ATACCTTCGGTA249640.24018698485573148No Hit
TCTACGGAGAGC242770.23357712831848232No Hit
CTTACACCAAGT241520.23237446155406288No Hit
GTGGGATGTTTC237020.22804486120215298No Hit
GCTTCGGTAGAT235010.22611097304496655No Hit
GTCTACACACAT206800.19896918950554907No Hit
CTCGAGAGTACG205190.19742015471297683No Hit
TAGCGGATCACG198350.19083916217807378No Hit
TGACCAATCTCG195350.18795276194346716Illumina PCR Primer Index 4 (100% over 12bp)
CCATACATAGCT194730.18735623922831512No Hit
CCTAGTACTGAT182810.17588760896281153No Hit
ACAAGGAGGTGA180690.17384788613035618No Hit
GGTCAGCTTAAC177430.17071133120875034No Hit
GCATGGCTCTAA177350.1706343605358275No Hit
GACTCTTGGCAA175360.16871971504687178No Hit
AATGGAGCATGA162130.1559906900122566No Hit
CGTCCGAAATAC135570.13043642660187277No Hit
ACGGCATGGCAT130410.12547181819834938No Hit
AGTTCCCGAGTA126310.12152707121105369No Hit
GAATGATGAGTG116640.11222324112150504No Hit
CTTAGTTCGCGT116210.11180952375454477No Hit
CCATGCGCTAAC116010.11161709707223764No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTG953.4290952E-66.00000051
AGGTCAC1001.2863038E-66.04
TCCGCGT1405.18412E-106.06
GGTCGCG2950.06.05
CATGCGT1303.6452548E-96.02
GACACTG2600.06.04
TGGGTTA600.00339626066.03
CAGTTCG909.150626E-66.03
TAGGTCG3250.06.03
GCTATTT550.0091600086.06
TAACGCC650.00126183846.01
GACCCGT32800.06.06
GGCCAGG1156.82503E-86.05
GCCTGGG600.00339626066.05
CTCGGAG550.0091600086.05
AACGCTG806.538364E-56.02
TTGACCG1001.2863038E-66.04
GTCCGTG5200.06.04
GTTCCGT1351.375156E-96.05
ACGCTGG751.7511232E-46.03
Produced by FastQC (version 0.11.2)