FastQCFastQC Report
Fri 11 May 2018
000000000-BGWPT_l01n01.331000000db06e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BGWPT_l01n01.331000000db06e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10393569
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA230746622.200901345822594No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA108472210.436472784276509No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA10072449.691031059687004No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA4871824.68734079698706No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGAGCGTA3469133.3377658819602773No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA2871162.7624389658643724No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCA2800982.6949164430428083No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA2357222.2679601203397985No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1972481.897788911585616No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1917061.8444674779183168No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA1197041.1517121789444993No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1098641.0570382512494025No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA772640.7433827590888173No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA753860.72531389362018No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA625450.6017663422449017No Hit
TACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCA577940.5560553838628483No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA522910.5031091822260476No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGCGCGTA495400.47664089207470506No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA466970.4492874391847497No Hit
TACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTTCGTA466070.4484215191143677No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA416510.40073818723866655No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATGACTGGGCGTAAAGGGCACGCA382110.36764079788184406No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGAGTA330910.31837956721122457No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA232950.22412897821720335No Hit
TACGTAGGGTGCGAGCGTTAATCGGCATTACTGGGCGTAAAGCGTGCGCA226330.2177596550328381No Hit
TACGTAGGGTGCGAGCGTTAATCGGACTTACTGGGCGTAAAGCGTGCGCA217740.20949492902774783No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA203360.1956594505698668No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA171400.16490966673719104No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA167990.16162879180385487No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA162820.15665456206621614No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA162220.15607728201929483No Hit
TTCCAGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA149290.1436368970081403No Hit
TACGTAGGTGGCAAGCGTTGTCCGGCATTATTGGGCGTAAAGCGCGCGCA147150.14157793150745426No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGTA116030.11163633974046835No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGTA112650.10838432880947825No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCATAAAGGGTGCGTA110070.10590202460771656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG1466500.0244.816241
GTGCGAG2732000.0244.744439
TAGGGTG2630750.0244.725285
TACGTAG6528250.0244.686611
GGTCCCG58350.0244.580127
ACGGAGG1464250.0244.556612
GTAGGGT2634400.0244.516394
AGGATGC244250.0244.297856
ACATAGG126550.0244.12882
ACGTAGG6534600.0244.019582
TAGGTCC58750.0243.957445
AGGTCCC59800.0243.566076
GGGTGCA1405350.0243.038767
CGTAGGG2942250.0242.88083
CGGAGGG1357050.0242.72523
TCAAACA610900.0242.3932245
TACATAG127300.0242.305571
ATAGGAT64650.0241.778824
GGGGCTA175750.0241.514958
GGTGCAA1453700.0241.06478
Produced by FastQC (version 0.11.2)