FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg010.351000000c734e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg010.351000000c734e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750727
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC31610.18055356431927994No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT29800.1702150021105518No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT29420.16804447523800112No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT28960.16541699533965032No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG28360.1619898476461493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG342550.094.87521
ATCGCGC343800.094.8480152
TAAGTAG327200.094.840321
TACACGA328900.094.768932
TCGTTAC342950.094.69535
ATACACG329150.094.6825261
ATGATCG362900.094.659696
GATCATG362850.094.6465452
ACACGAT329100.094.624733
ATCGTCT363850.094.5822459
ATCATGA363350.094.5685963
CGATCGT343900.094.558012
ATCGTTA343900.094.54424
CGATCAT363450.094.52951
GATCGCG345000.094.518111
TCATGAT363900.094.490944
GATCGTC363500.094.477318
TTACCAT342700.094.473318
CACGATC329350.094.466384
CATGATC363850.094.464755
Produced by FastQC (version 0.11.2)