FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg009.351000000c7331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg009.351000000c7331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592419
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT28740.18048013745126126No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT27800.17457716844624438No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC27440.1723164569124081No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG27080.17005574537857185No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT26650.16735545104648966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGC308600.094.85992
TAAGTAG296200.094.7258151
ATGATCG311700.094.6479346
TACACGA309800.094.645792
TCGTTAC308350.094.644135
ATACACG310350.094.60051
ACACGAT309800.094.569133
GATCGCG309750.094.569061
AAGTAGA296850.094.5183942
ATCGTCT312350.094.511819
GATCGTC312250.094.511648
TTACCAT308700.094.44458
CGATCAT312750.094.406111
TCGCGCG310150.094.401153
GAGTCTT297300.094.391317
CATGATC312950.094.391315
CACGATC310200.094.355314
ATCGTTA309100.094.353024
GATCATG313200.094.34632
CGTTACC308700.094.33686
Produced by FastQC (version 0.11.2)