FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg008.351000000c7324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg008.351000000c7324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495785
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT25640.1714150095100566No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC25610.1712144459263865No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG25300.16914195556179532No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT24650.16479641124894287No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT24270.16225593918912143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTAG281900.094.831271
ATCGCGC291350.094.8204352
TACACGA291950.094.739452
ATACACG292100.094.723311
ATCGTTA303450.094.671044
ATGATCG291500.094.657576
TCGTTAC303450.094.6240845
ACACGAT292300.094.5935063
GATCGCG292100.094.576961
TTACCAT303700.094.4992758
GATCGTC292200.094.4958348
CGATCGT303700.094.4679952
TACCATC303400.094.4361659
CATGATC292200.094.430815
CACGATC293100.094.416354
ATCGTCT292950.094.4160469
AAGTAGA283150.094.395852
GGTTCTT292050.094.34929
GATCATG292550.094.317842
TCGCGCG292550.094.317843
Produced by FastQC (version 0.11.2)