FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg007.351000000c7317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg007.351000000c7317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213944
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC24660.20313951879164113No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT24130.19877358428395378No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG23070.1900417152685791No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT23030.1897122107774329No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT23020.18962983465464633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCG156.618223E-494.999266
ATCTTTT254.6210334E-794.999268
TCGATCG242400.094.84251
ATCGCGC238350.094.81992
TAAGTAG232200.094.79471
TCGTTAC243050.094.686575
GATCGCG239350.094.661891
TACACGA234950.094.635352
ATACACG234800.094.614881
ATCGTTA243500.094.6091164
ATCGTCT241950.094.606629
TCATGAT241850.094.5868154
ATGATCG241200.094.585716
TAGAGTC233450.094.551635
AAGTAGA232800.094.550382
TTACCAT242750.094.529648
ACACGAT235100.094.514363
GATCGTC241800.094.4688648
CATGATC241750.094.4491045
ATCATGA242100.094.410673
Produced by FastQC (version 0.11.2)