FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg006.351000000c730a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg006.351000000c730a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345189
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC26940.20026925584434602No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT26160.19447081413838502No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT25280.1879289824701213No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG24450.18175884578300894No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT24000.1784135909526468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTTT301.2321834E-894.999739
ATCGCGC264700.094.910022
TCGATCG269500.094.841111
TAAGTAG251500.094.810881
ATACACG262200.094.7098851
TACACGA262050.094.7097242
TCGTTAC270000.094.665485
GATCGCG265800.094.66021
ACACGAT262300.094.637563
ATGATCG273650.094.548436
AAGTAGA252200.094.5100562
CGATCGT270550.094.490592
TTACCAT270350.094.472658
ATCGTCT273950.094.4622349
TAGAGTC252700.094.454635
ATCGTTA271100.094.439064
GATCGTC274050.094.4277658
TCTCTTG263600.094.42319
GATCTCT263400.094.422677
CACGATC262950.094.421694
Produced by FastQC (version 0.11.2)