FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg005.351000000c72fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg005.351000000c72fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248277
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT23560.18874015943576627No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG23290.18657717798213055No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC23140.18537552161899964No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT22860.1831324297411552No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT22800.18265176719590284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCG156.618217E-494.999446
TCGATCG244350.094.8828051
TAAGTAG237300.094.819291
TACACGA241200.094.7828142
AAGTAGA237300.094.759252
ATCGCGC253200.094.736812
ATACACG241600.094.704541
ATCGTTA244600.094.591634
TCGTTAC244250.094.591055
ACACGAT241400.094.5468753
ATGATCG249200.094.522926
CGATCGT245300.094.495982
GATCGCG253350.094.493231
TTACCAT243950.094.4737248
CGTTACC243750.094.434326
CACGATC241400.094.428824
ATCATGA249400.094.428073
CATGATC249300.094.427845
TCATGAT249800.094.4099664
GATCGTC249300.094.408798
Produced by FastQC (version 0.11.2)