FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg004.351000000c72ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg004.351000000c72ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240401
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT26500.21364058880958656No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT24910.2008221534810114No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG24680.1989679144083244No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC24530.19775862805657204No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT23670.19082538630652507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTT353.3105607E-1094.99945
TAAGTAG228200.094.8745041
ATCGCGC245250.094.863822
TCGATCG248800.094.846661
ATCGTTA249450.094.7518544
TCGTTAC249350.094.751765
ACACGAT242550.094.725233
GATCGCG246350.094.6716161
ATACACG242550.094.666471
TACACGA242950.094.588822
CATGATC251250.094.545675
ATGATCG251050.094.545316
TTACCAT249500.094.542488
GATCGTC251350.094.508058
CACGATC242800.094.4907464
ATCGTCT252050.094.490579
AAGTAGA228950.094.4599842
ATCATGA252250.094.4533163
TCATGAT251850.094.4335864
CGTTACC249450.094.428146
Produced by FastQC (version 0.11.2)