FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg003.351000000c72d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg003.351000000c72d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324243
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC27440.20721272455281997No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT25910.19565895383249146No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG25400.19180769692571528No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT25200.19029740009952856No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT24620.18591753930358704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCG301.2321834E-895.06
TAAGTAG253200.094.849931
TCGATCG265350.094.820991
ATCGCGC259250.094.798462
ATACACG257150.094.722921
AAGTAGA253600.094.700322
TACACGA257600.094.686532
TCGTTAC265800.094.642595
ATCGTTA266050.094.625074
ATGATCG265850.094.6247946
ATCGTCT266750.094.5548259
CGATCAT265950.094.553491
ATCATGA265900.094.5534063
ACACGAT257850.094.539463
TTACCAT266500.094.536588
GATCGCG260600.094.489651
CGATCGT266400.094.4650962
GATCATG266000.094.464282
TACCATC266500.094.447479
CATGATC266200.094.446845
Produced by FastQC (version 0.11.2)