FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg002.351000000c72c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg002.351000000c72c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293425
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC25310.19568200707424085No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT24660.19065659006127142No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT24570.1899607630902449No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG23500.18168815354581827No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT22810.1763534801012815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGC256000.094.813532
TAAGTAG248400.094.8078461
ATGATCG263100.094.800486
ATCGTCT263650.094.782889
GATCGCG256200.094.758051
GATCGTC263500.094.7467048
ATACACG245050.094.72771
TCGATCG257350.094.722211
ATCGTTA257400.094.648464
TCGTTAC257150.094.629645
TACACGA245200.094.57292
TAGAGTC249400.094.484845
CGATCGT258050.094.483672
CATGATC264300.094.477895
TTACCAT257950.094.446658
ACGATCT245550.094.4380955
AAGTAGA249200.094.4272462
CGTTACC257050.094.426236
ACACGAT245550.094.4187553
ATCATGA264400.094.406223
Produced by FastQC (version 0.11.2)