FastQCFastQC Report
Tue 6 Mar 2018
000000000-BDTBB_l01n01_gg001.351000000c72b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDTBB_l01n01_gg001.351000000c72b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631155
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTAG317350.094.894711
ATCGCGC312950.094.862872
TCGATCG306350.094.8599241
GATCGCG313850.094.666511
ATGATCG325050.094.663386
GATCGTC324750.094.663068
ATACACG300150.094.651321
TCGTTAC308150.094.567885
ATCGTTA308600.094.522324
ATCGTCT325550.094.517989
TACACGA300900.094.431182
AAGTAGA318650.094.418122
CATGATC326200.094.373335
CGATCGT308250.094.367682
CGATCAT325850.094.358081
ACACGAT301250.094.353013
CACGATC301350.094.321694
TCGCGCG314500.094.319833
ACGATCT301500.094.306285
TTACCAT308200.094.290538
Produced by FastQC (version 0.11.2)