FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n02_trinidad8.352000000998e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n02_trinidad8.352000000998e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502504
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT1205.175234E-616.8754
CTATACT6700.016.4552254
GCTATAC3200.016.1718773
CTACACT4750.016.1052654
TAGACAG2800.016.0714285
TACACTG5000.015.7499995
TCTATAC4350.015.5172413
CTATACG1351.5821943E-514.9999994
GTATAGG4550.014.8351641
ACAGTCC2304.2382453E-1014.6739128
TACACAG4600.014.6739125
GTCCAGG3850.014.610391
GTGTTAG3100.014.51612951
CTTACAC2752.910383E-1113.9090913
TCAGGAC2306.673872E-913.6956513
CCTAGAC2703.2923708E-1013.3333333
TAGACCG2205.5048076E-813.2954555
GTCTAGA3101.6370905E-1113.0645171
CAGGACA3101.6370905E-1113.0645174
ACTGTGC4200.012.8571428