FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n02_trinidad5.352000000998d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n02_trinidad5.352000000998d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168859
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG7050.016.9148941
GTGTAGG6350.016.2992131
TACACAG6050.014.8760345
GTCTACG5000.014.8500011
CCAGGAC4900.014.2346943
TGTAGGG4650.014.0322572
TATACTG7650.013.8235295
CCTATAC6050.013.7603313
GTGTTAC4450.013.6516861
ACTGAGC5050.013.3663368
TACACTG5250.013.2857155
GGACCGT3400.013.2352956
GTGTATA7350.013.1632651
GTCCAGG5350.013.0373831
GTTCTAG4950.012.7272721
TATTGGG9050.012.6795572
GTATTGG9600.012.6562491
GCCTAGG4100.012.6219511
GTATAGA7350.012.5510211
GTCCTGG5600.012.4553571