FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n02_trinidad4.352000000998c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n02_trinidad4.352000000998c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189674
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC4150.014.0963853
TCTACAC7100.013.9436623
GTATTGG9550.013.9005241
ACCGTGC6600.013.6363638
GGACCGT3650.013.5616446
TACACCG4350.013.4482765
GTGTAGG5050.013.3663361
TAGACTG4250.013.2352935
CCTAGAC3800.013.0263163
GTCTTAG3800.013.0263161
GTGTAAG4150.013.0120481
TATACTG7450.012.9865775
TACACAG6250.012.965
GTCTAAG3303.6379788E-1212.9545451
GTCCAGG4350.012.9310341
TACACTG6800.012.9044125
GTGTATG8500.012.7058821
CCTATAC6050.012.6446283
GTGTTAG5000.012.61
GTATAGA6800.012.573531