FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n02_trinidad1.352000000998bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n02_trinidad1.352000000998bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264785
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG5900.014.4915261
TATACAG7200.014.3755
TAGGCTG4800.014.0625015
TACACTG6800.013.8970595
CTACACT6200.013.4274194
CCTATAC7650.013.2352943
TAGACTG4600.013.2065215
GGACAGT3950.013.1012666
GGACCGT2754.4019544E-1013.0909096
TATACCG4500.013.05
CCTACAC5400.012.9166673
CCTAGAC4900.012.8571423
TAGAGTG4800.012.6562515
AGGACAG4450.012.640455
GTCCTAG5350.012.6168221
TATACTG7850.012.6114655
TAGGACC3750.012.64
ACTGTCC4700.012.4468098
CTAAGAC3457.2759576E-1212.3913053
CGTACAC4550.012.3626373
Produced by FastQC (version 0.11.2)