FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n01_trinidad9.351000000998f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n01_trinidad9.351000000998f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354075
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG5250.017.1428595
GTGTAGG6450.016.3953481
TAGACTG5050.016.0396045
ACAGTCC3200.015.4687518
ATTCGCG3800.014.80263137
GTTACAC3700.014.5945963
GTATTGG10350.014.5652181
GTAGGAC3900.013.8461533
GTGCTAG5200.013.8461531
GTGTATG8700.013.7068971
TATTGGG10250.013.6097562
GTATAGG8450.013.5798821
TACACTG7100.013.3098595
CTACACT6350.013.1102364
ACTGTGC5850.013.0769228
TAGGACA6750.013.04
CCTATAC5750.012.9130433
ATTGGGT9950.012.8894473
CCTACAC4750.012.7894743
CTTACAC3700.012.7702713