FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n01_trinidad8.351000000998e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n01_trinidad8.351000000998e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502504
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG7150.017.6223771
TAGACTG2800.016.8755
TCTACAC3850.016.3636363
CCCTTAC1803.912646E-916.251
CAGGACC2800.016.0714284
CGCTATA1552.0672633E-715.9677432
GTATAGG4800.015.9374991
TATTGGG7350.015.9183672
GTCGTAA1602.9647344E-715.4687511
CGTTCGT4900.015.15306111
TAGACAG3000.015.0000015
TCGCGCG4750.014.68421239
ATTGGGT6500.014.5384623
CCGCACT1553.618934E-614.51612954
ACTGTCC3000.014.2500018
GGAGTGT3950.014.2405076
GTCCTAG2551.3278623E-1014.1176471
CTGTCCG1605.028629E-614.0625019
AGACTGT2408.039933E-1014.0624996
GTTCGTG5650.013.93805212