FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n01_trinidad5.351000000998d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n01_trinidad5.351000000998d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168859
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG8950.015.5865921
ACTGTGC5550.015.4054058
TAGACTG3850.014.6103895
GTATAGG7200.014.3751
GCTACAC6400.014.06253
TCTACAC5650.013.9380533
GTATTAG5500.013.9090911
TACACAG6350.013.8188995
GTATAGA7250.013.6551721
CTACACT6050.013.3884314
CTACACA7250.013.3448274
GACTGCG2401.2134478E-813.1249997
CTATACT8950.013.0726254
CCTATAC5900.012.9661023
ACCGTGC6800.012.9044128
GTCCTGG4950.012.7272721
TAAGACT4250.012.7058824
TGTACTG8550.012.6315795
TCTATAC6100.012.5409823
TACACCG4850.012.5257745
Produced by FastQC (version 0.11.2)