FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n01_trinidad4.351000000998cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n01_trinidad4.351000000998cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189674
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG5700.016.184215
TAGACTG3600.015.6255
CCAGGAC4100.014.2682933
CCTATAC5800.013.9655173
TAGACGG2102.993511E-813.9285725
TACACTG6650.013.8721815
ACCGTGT4550.013.8461538
TACACCG4550.013.8461535
CTACACC7500.013.84
GTATTGG7250.013.6551721
CCTACAC5950.013.6134453
GTATTAC5050.013.3663361
GCACCGT4050.013.3333346
GCTACAC7100.013.3098583
GTTCTAG4450.013.1460681
CCGTACG2257.392009E-813.09
CGTACAC3650.012.9452053
CTAGGAC5600.012.8571433
GTCCTAG4550.012.8571421
ACAGTGC7200.012.81258