FastQCFastQC Report
Tue 15 Aug 2017
000000000-BDGGG_l01n01_trinidad1.351000000998bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BDGGG_l01n01_trinidad1.351000000998bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264785
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG4750.016.1052635
TATACTG7600.015.3947365
TACACAG6650.015.2255655
ACCGTCC3550.015.21126658
GTATTGG8450.014.91124251
ATTCGCG4250.014.8235337
TACACTG6450.014.6511625
TATTGGG8600.014.3895352
GTGTAGG6100.014.3852461
GTATAGG6900.014.3478261
CCTACAC6200.014.1532263
CTACGCT2408.058123E-1014.0625014
TTCGCGC4200.013.92857238
GTGTACG4700.013.8829781
GTCCTGG4900.013.775511
ACAGTGC6950.013.5971238
CCTATAC6050.013.3884293
CGTTCGT5600.013.25892811
TAGCACT6300.013.2142854
GTATAGA5800.013.1896561