FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_prvabc59b1-1000.3520000009e935.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_prvabc59b1-1000.3520000009e935.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343374
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCGCCTGGG36990.1578493232407631No Hit
GCCGGGGACTGTTTCCAGTGACTTCCGCATCTTTTTCCCATGTGATGTCA35510.15153364337062714No Hit
GCCACATCCAAGATCAATGACCTTTCCATAGGGCTGCAGGTATCCCCGCT35110.14982670286518499No Hit
CTTTTCTCTCTTATTTCCCATCATGTTGTACACACAGCTCTGGCACTCTC33370.14240151166651163No Hit
GCCACATCGAGCCGGGGACTGTTTCCAGTGACTTCCGCATCTTTTTCCCA32370.13813416040290624No Hit
GCCGGAGACCCTAGAGACCATAATGCTTTTGGGGTTGCTGGGAACAGTCT29600.12631359740271933No Hit
CCGGGGACTGTTTCCAGTGACTTCCGCATCTTTTTCCCATGTGATGTCAC29570.12618557686481116No Hit
GCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCA29020.12383853366982822No Hit
GAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTG28170.12021128509576363No Hit
GCTGCACCTCGCTGTGCCCATCCCAGGCGGCATCTAGCTTCCATGGACCA24120.10292851247816182No Hit
GCGGCATCTAGCTTCCATGGACCACAGTATGACACCAGATCCTGCTTGAC24000.1024164303265292No Hit
CTGGAATCTATGACACGGTCAGCTTTAAAGTTGGCGCCCATCTCTGAAAT23970.10228840978862101No Hit
GGGTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACA23860.10181900114962443No Hit
CTTTTCCAACCACATTGGGGATGAATAAGTGTTATATACAGATCATGGAT23860.10181900114962443No Hit
CAGAAAGCAACTTAGTAAGGTCAATGGTGACTGCAGGATCAACTGATCAC23770.10143493953589995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA8900.0123.820222
GATCGGG10700.0107.0560761
ATTGGGA3650.0101.301382
TCGGGAG10650.095.305173
AAGCGGC2150.094.418619
GAACGGG5550.092.747741
AATCGGA406.1200444E-790.6252
AGAGCGG8350.088.5628748
AACCGGA451.2334058E-680.555552
CTCTTAT8750.073.742868
TCGGGAA3350.073.582093
TCTCTTA8900.072.57
TCTTATT9000.071.694449
GAACGGA1550.070.161291
CGGGCAT1450.070.01
ACGGGAA2050.067.195123
GAGAGGG23850.065.6603857
GATCCGG1550.065.483871
TTCGCCG1009.094947E-1265.25145
TCGGAGT451.194192E-464.444441
Produced by FastQC (version 0.11.2)