FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_prvabc59b1-100.3520000009e942.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_prvabc59b1-100.3520000009e942.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63136
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC12842.033705017739483Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATA1050.16630765331981753Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATT770.1219589457678662Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGGAGATC750.11879118094272681Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCAG100.0070752385145.09
GACGGAA100.0070752385145.02
ATTGGGA2100.0138.095232
ATCGGGA6250.0138.042
CGGGAGA9850.0136.167514
ACGGGAG3750.0135.333333
TCGGGAG6750.0132.111113
GAGGGGC10500.0131.880959
AAGGGGC2650.0131.320769
GACGGGA1050.0131.190482
GAACGGA1850.0129.324331
GAACGGG3650.0129.109571
AAGCGGC900.0128.888899
AACGGGA3600.0128.888892
ATGGGAG1650.0127.424243
AGAGCGG4550.0125.879138
GAGCGGC3750.0125.666669
GAGAGCG4150.0124.036147
GGAGAGC4050.0123.518526
GATCGGG7300.0123.150681
Produced by FastQC (version 0.11.2)