FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_prvabc59b.3520000009e81c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_prvabc59b.3520000009e81c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981387
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC80900.27135021384342256Illumina Single End PCR Primer 1 (97% over 35bp)
GCCACATCCAAGATCAATGACCTTTCCATAGGGCTGCAGGTATCCCCGCT36150.1212522896222463No Hit
GGAGGGGGCAACCATAGGATTCTCAATGGACATTGACCTGCGGCCAGCCT33880.11363838374555198No Hit
GGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTC31460.10552135633515541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA52550.0136.308272
TCGGGAG51050.0129.946123
GATCGGG57950.0126.859361
ATTGGGA22950.0126.045762
GATCCGG20600.0124.235441
GAGGGGC49450.0110.5460059
GAACGGG14450.0105.363331
CGGAAAG13350.0105.355815
GAACCGG4200.0105.2976151
AAGCGGC8150.0104.079769
ATTCGGA7800.0102.243592
AGAGCGG32600.099.85438
TCGGGAA21150.099.408983
AACCGGA4200.096.6666642
GAGAGGG87550.096.1422047
GATTCGG9700.095.6701051
CGGGAAA23700.090.5485154
GATCCGA3450.088.2608641
GGAGAGG98400.088.19366
ATCCGAA3200.086.093752
Produced by FastQC (version 0.11.2)