FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_negative.3520000009e893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_negative.3520000009e893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171735
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC23571.372463388359973Illumina Single End PCR Primer 1 (97% over 35bp)
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA1890.11005327976242466Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA23650.0141.01482
ATCCGGA8100.0140.524692
TCGGGAG23400.0137.873933
TCCGGAG7450.0135.268453
GAGCGGC8900.0134.410139
CGGAGAG11650.0133.175965
CGGGAGA25150.0132.31614
TCCGGAA3600.0130.902773
GATCCGG9000.0130.51
ATTGGGA9200.0129.239142
GAGGGGC20550.0129.124089
AAGGGGC5450.0129.03679
GATCGGG27050.0128.650651
ATTCGGA3750.0127.599992
AACGGGA4900.0127.2448962
AAGCGGC2800.0126.8759
CCGGAGA8450.0126.124264
GAGAGCG15150.0124.900997
AGAGCGG13650.0124.285718
GGAGAGC15650.0124.153366
Produced by FastQC (version 0.11.2)