FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_mr766a1-1000.3520000009e95f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_mr766a1-1000.3520000009e95f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055356
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC28960.27440977262648814Illumina Single End PCR Primer 1 (96% over 33bp)
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA23440.22210514745735088No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG21960.20808144360765468No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA19350.18335045235920389No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA16250.15397647807943482No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC14650.13881571716084432No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA14640.1387209624051031No Hit
GGCCAGTGCATGGCTCATGTGGCTTTCGGAAATTGAACCAGCCAGAATTG14050.13313043181637285No Hit
GCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTTC13330.12630808940300714No Hit
GCGGGGTCAACCGATCATATGGACCACTTCTCTCTTGGAGTGCTTGTGAT12290.11645359480592331No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA12220.11579031151573496No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG12200.11560080200425259No Hit
GGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCC11940.11313717835498163No Hit
GCGGGAGTGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCC11400.10802042154495735No Hit
GCGGAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGG11220.1063148359416159No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG11200.10612532643013352No Hit
GGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTT11140.10555679789568638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGA4900.0131.683672
TCGGGAG28600.0129.029723
GATCGGG33450.0124.192831
GATCCGG10000.0123.9751
GAGGGGC25950.0114.8265849
AAGGGGC9250.0111.2972959
GGAGAGC21250.0109.517656
GAGAGCG21000.0108.059527
TTCGGAG5850.0104.102563
GAACCGG2750.0102.8181841
CGGGAGA40100.099.077314
GGGAGAG57050.095.056975
CGTAGAG1300.094.8076865
ATCGGGA43150.093.586332
GGAGAGG51550.093.103796
GAGGGTC4700.091.010649
AGAGCGG22750.087.956048
GAGCGGC19050.084.488189
ACGGGAG8000.084.281253
CGGAGAG23100.081.915585
Produced by FastQC (version 0.11.2)