FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_mr766a.3520000009e850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_mr766a.3520000009e850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997365
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC58020.29048271097170525Illumina Single End PCR Primer 1 (96% over 33bp)
GGTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGAC33030.16536787217158608No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT31700.1587090992382464No Hit
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA28550.1429383212382314No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA28060.1404850891048957No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT25590.12811879651440775No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG25360.1269672793905971No Hit
GGCGGCTACCTTATCGGCCTCAGGCCGATAGAGCGAGGCTATGAGGCCAT24660.12346266205726043No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA23400.11715435085725442No Hit
GGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCT23250.1164033614286823No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA21260.10644023500962518No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA21150.10588950942867227No Hit
GGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTACTCCATC21140.10583944346676748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAG51250.0133.117083
GATCGGG63150.0127.090271
GATCCGG23800.0125.5041961
ATTCGGA8850.0118.785312
GGAGAGC45900.0111.9880146
GAGAGCG43050.0111.655067
TTCGGAG10200.0105.1960753
GGGAGAG102100.0104.311955
CGGGAGA74900.0101.82914
GGAGAGG87450.0100.148666
ATCGGGA81200.095.803572
GAACCGG6850.095.255481
ACGGGAG16350.094.449543
AAGGGGC24750.093.444449
GAGGGGC58100.093.214299
CGGAGAG40600.092.142865
GATTCGG12450.090.843371
GCTCCGG8100.087.716051
GAGCGGC36900.086.449879
GATTCGA2650.084.811321
Produced by FastQC (version 0.11.2)