FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_67n2.3520000009e8f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_67n2.3520000009e8f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453144
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA7250.0114.02
GATCGGG7850.0109.904461
GAACGGG5250.096.666671
CGGGAGA12300.096.077244
GAGCGGC5900.090.93229
ACGGGAG5000.089.93
GAGGGGC15150.087.574269
AGAGCGG7300.085.4109658
AACGGGA5550.083.603612
GAGAGGG19500.082.538477
TCGGGAG11050.082.013573
GAGAGCG7100.080.6690147
GATCCGG1400.077.678571
GGGAGAG24600.076.331315
GGAGAGG21550.073.67756
CGGAGAG4050.062.654325
GATTGGG6200.061.9758031
CGGGAAA3200.061.1718754
GGAGAGC9900.060.050516
ATTGGGA6350.058.2283482
Produced by FastQC (version 0.11.2)