FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_67n1.3520000009e886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_67n1.3520000009e886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331010
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC38671.1682426512794175Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAG13400.0137.425373
ATCGGGA19900.0136.984922
CGGGAGA32350.0132.8984
AACGGGA12500.0131.079992
GAGGGGC35600.0128.911519
TCGGGAG22850.0128.18383
GAGCGGC12300.0124.959359
ATTGGGA10750.0122.744192
GAACGGG13750.0121.81
GAGAGCG15650.0118.594257
AGAGCGG17400.0116.249998
ACGGAAG6950.0114.748213
GACGGGA4750.0114.473692
GATCGGG24600.0112.876021
AAGGGGC9950.0112.211069
CGGAGAG4700.0111.0638355
CCGAAGA2100.0110.476194
TCGGGAA4950.0109.848483
AAGCGGC4000.0108.759
GGAGAGC17750.0108.647886
Produced by FastQC (version 0.11.2)