FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_66n2.3520000009e91b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_66n2.3520000009e91b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583423
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC26140.4480454147333924Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA21650.0126.247112
GATCGGG22500.0122.444451
TCGGGAG23150.0118.066953
GATCCGG9050.0114.558011
CGGGAGA28350.0108.686074
GAGAGCG17250.0102.550727
GAGCGGC11950.099.497919
GGAGAGG35650.099.2426456
GAACGGG8050.099.068331
ATCCGGA10050.098.830852
AGAGCGG15550.096.977498
TTCGGAG3850.096.038963
GAGAGGG36250.095.27
CGGAGAG13450.094.330855
ATTGGGA8850.093.389832
ATTCGGA4050.093.086422
GGAGAGC20450.092.8850866
TCCGGAG10100.092.5990143
CCGGAAA5350.092.149534
GATTCGG4800.092.1354141
Produced by FastQC (version 0.11.2)