FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_66n1.3520000009e928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_66n1.3520000009e928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463464
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA24290.5240968014775689No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG21770.46972364628104885No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC21340.4604456872594203No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC21300.4595826213039201No Hit
GCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA20360.43930057134966255No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC18870.4071513645072756No Hit
GGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGG18860.4069355980184005No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG18810.40585676557402517No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG16160.34867864602212906No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA16040.34608944815562803No Hit
GTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA15520.3348695907341239No Hit
TGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACG14470.3122141094022405No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC14220.30681994718036354Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTA14180.3059568812248632No Hit
GCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGT13420.28955862807035715No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA13370.28847979562598175No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT13190.2845959988262303No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG12610.27208154247147565No Hit
GTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTAC12120.26150898451659677No Hit
GTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCG11830.2552517563392194No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA11760.25374139091709386No Hit
GGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGA11700.2524467919838434No Hit
CTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAAC11500.24813146220634183No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCAC11260.2429530664733399No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT11020.23777467074033798No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT10720.23130167607408558No Hit
GGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGT10560.22784941225208433No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC10040.21662955483058016No Hit
CCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGAGAACCA9960.21490342291957953No Hit
GTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA9860.21274575803082874No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT9780.21101962611982808No Hit
GCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCA9730.2099407936754527No Hit
GGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAA9380.20238896656482488No Hit
GTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTA8930.1926794745654463No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC8820.1903060431878204No Hit
CCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGGCGGTC8650.18663801287694406No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT8510.18361728203269292No Hit
CTGGTATCTTCGACTGATTTCAGCTCCATCCGCAAGGGACTTCACCTACA8300.17908618576631627No Hit
GTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGG8150.17584968843319007No Hit
GCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA8120.17520238896656484No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTG7620.16441406452281082No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT7470.16117756718968462No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAG7410.15988296825643417No Hit
CACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGG7350.15858836932318368No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGG7240.1562149379455578No Hit
CCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCG7220.15578340496780765No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT7190.1551361055011824No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACCGACGCT7060.15233114114580637No Hit
CGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCC6940.1497419432793054No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG6780.14628967945730412No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA6730.14521084701292872No Hit
GTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTC6630.14305318212417792No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA6550.1413270502131773No Hit
GTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGT6490.14003245127992683No Hit
CTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGGCG6290.1357171215024252No Hit
CGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGG6240.1346382890580498No Hit
TCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCG6130.13226485768042395No Hit
CTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGT6070.13097025874717344No Hit
GCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCC5910.12751799492517218No Hit
GTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA5870.12665492896967187No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA5870.12665492896967187No Hit
GGCCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTG5860.12643916248079679No Hit
CCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTTT5820.12557609652529647No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA5820.12557609652529647No Hit
CCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCC5760.12428149759204597No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA5730.12363419812542074No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC5710.12320266514767059No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTA5630.12147653323666995No Hit
CGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTC5620.12126076674779486No Hit
CTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGA5560.1199661678145444No Hit
CGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCGCA5530.11931886834791915No Hit
CGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTC5460.11780850292579359No Hit
GAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTAT5420.11694543697029326No Hit
GAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGT5380.11608237101479295No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC5340.11521930505929262No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTG5270.11370893963716708No Hit
GGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCT5240.11306164017054184No Hit
GCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGC5160.1113355082595412No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC5110.1102566758151658No Hit
GTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCC5090.10982514283741564No Hit
CGTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGC5060.10917784337079038No Hit
CCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGT4990.10766747794866482No Hit
CAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGC4930.10637287901541437No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4900.10572557954878913No Hit
GTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCG4870.10507828008216387No Hit
CCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACC4850.10464674710441373No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG4810.1037836811489134No Hit
CGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATCACTATGACCTGC4770.10292061519341308No Hit
CGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTG4760.102704848704538No Hit
CCAGCCTACACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCT4660.1005471838157872No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC4660.1005471838157872No Hit
GGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCA4640.10011565083803703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGGA9950.0133.34172
ATCGGGA16950.0131.740422
GATTGGG10850.0126.958531
GATCGGG19050.0121.4042051
GGAGAGG25950.0118.458576
GAGAGGG26100.0115.8333367
TTGGGAG10050.0111.094533
TAGGGAG3800.0110.65793
GGGAGAG35200.0108.3380665
GAGAGCG9550.0106.282737
AGAGCGG9750.0104.8461468
GAGCGGC8150.0101.411039
GGAGAGC10400.0100.384616
AACGGGA5750.099.6086962
TCGGGAG21550.088.480283
TCGGGAA5100.085.294113
GAGGGGC28400.084.7535259
TTGGAAG2700.077.870373
CGGAGAG4450.076.573045
GAGCGGT1900.076.315799
Produced by FastQC (version 0.11.2)