FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_65o1.3520000009e992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_65o1.3520000009e992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425428
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC7310.17182696014366708No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC6180.14526547382870897No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC5680.13351260377784255No Hit
GGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGG5680.13351260377784255No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC5540.13022180016359994No Hit
GCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA5380.1264608817473227No Hit
GGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGA4840.11376778209238696No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG4710.11071203587916169No Hit
GCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGC4320.1015447972394859No Hit
TGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACG4270.10036951023439925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG4350.078.333331
GAGGGGC3750.063.8000039
GAGAGGG5350.063.6915867
ATCGGGA5300.061.5566062
GGAGAGG5450.061.192666
GGGAGAG7300.053.630145
GATTGGG2450.050.306121
AGAGGGG6900.047.282618
CGGAGAG2600.044.6153875
TCGGGAG6800.043.7132383
TCCGGAG2750.042.181823
GGGAGCG4050.041.172842
ATTGGGA2700.040.277782
GCTATAG2800.038.8392876
TTGGGAG3300.037.3484843
CTATAGT2950.036.8644077
TATAGTA2950.036.8644078
GCTCCCA2950.036.8644071
AAGGGGC1604.579124E-836.259
CTCCCAC4450.035.8426931
Produced by FastQC (version 0.11.2)