FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_65n2.3520000009e8d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_65n2.3520000009e8d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369583
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC13190.35688870970796815No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA11230.303855967401098No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG9770.26435198588679676No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG9550.2583993311380664No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA9230.24974092423082228No Hit
GCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA8970.24270596861868649No Hit
GTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCG8060.218083623976211No Hit
GGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGG7630.20644888969460176No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT7590.20536658883119624No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT6940.18777919980085664No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT6930.18750862458500525No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC6880.18615574850574837No Hit
TGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACG6840.18507344764234288No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA6810.1842617219947887No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA6600.17857964246190977No Hit
GCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGT6230.16856835947540877No Hit
GGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTA6110.16532145688519223No Hit
GTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTAC6020.16288627994252983No Hit
GTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA5980.1618039790791243No Hit
CTGGTATCTTCGACTGATTTCAGCTCCATCCGCAAGGGACTTCACCTACA5420.1466517669914471No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG5380.1455694661280416No Hit
CTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAAC5300.14340486440123057No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC5240.1417814131061223No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCAC5210.14096968745856817No Hit
GGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGA5200.14069911224271678No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG5090.13772278486835163No Hit
GTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGT5040.13636990878909475No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG4940.13366415663058095No Hit
CGTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGC4910.13285243098302682No Hit
GTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGG4870.1317701301196213No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT4550.12311172321237719No Hit
GGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGT4500.1217588471331203No Hit
GTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCG4220.1141827410892817No Hit
GCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCA4150.11228871457832205No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTG4100.11093583849906517No Hit
CCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTTT4000.10823008634055138No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA3990.1079595111247No Hit
GCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA3860.10444203331863208No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC3710.1003834050808614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC8050.0115.2794959
ATTGGGA3150.0108.174612
ATCGGGA8550.0106.842112
GATCGGG9150.0103.797811
CGGAGAG5500.096.227275
GATTGGG3750.094.733341
GAGAGGG13600.092.224267
GGAGAGG14300.088.7237856
TCCGGAG4450.087.977523
GGGAGAG15900.087.09125
TTCGGAG2250.083.777783
GAGCGGC5800.082.59
GGAGAGC7250.082.06
ATCCGGA4600.078.804352
GAGAGCG7050.078.156037
TCGGGAG12700.076.496063
GATTCGG2250.074.1111151
AGAGCGG7400.069.560818
ACTCGGA651.3956196E-766.923072
TTGGGAG5900.065.127113
Produced by FastQC (version 0.11.2)