FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_65n1.3520000009e8e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_65n1.3520000009e8e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382048
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC39391.0310222799229416No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA25770.6745225730798224No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG23300.6098710109724433No Hit
GCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA22650.5928574419968171No Hit
GGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGG22420.5868372560515955No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC17930.4693127565122707No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA17020.4454937599463942No Hit
TGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACG16010.4190572912304213No Hit
GCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGT15450.4043994471898819No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT15230.39864100845966993No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC15000.3926208225144484No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA14970.3918355808694196No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG14650.3834596699891113No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT14130.3698488148086104No Hit
GTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA13310.34838554317782056No Hit
GGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTA13260.34707680710277244No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG12770.3342511935673005No Hit
GGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGT12640.33084847977217524No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC12470.3263987771170115No Hit
GGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTGA11520.3015327916910964No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG11130.29132465030572074No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA10310.2698613786749309No Hit
CTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAAC10030.2625324566546612No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT9960.26070022614959376No Hit
GGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAA9580.25075383197922774No Hit
GTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCG9190.24054569059385206No Hit
CCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCAC9190.24054569059385206No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC8870.23216977971354386No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT8860.23190803249853423No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA8790.2300758019934668No Hit
GTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA8730.228505318703409No Hit
CTGGTATCTTCGACTGATTTCAGCTCCATCCGCAAGGGACTTCACCTACA8700.22772007705838007No Hit
CGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCGCA8680.22719658262836084No Hit
GTGCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGT8400.21986766060809113No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGG8370.21908241896306224No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC8270.2164649468129659No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT8240.21567970516793702No Hit
GTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTA8190.21437096909288886No Hit
GCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCA8090.21175349694279255No Hit
GTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTAC7900.2067802998576095No Hit
GTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGG7750.20285409163246504No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA7720.20206884998743613No Hit
GGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCT7210.1887197420219449No Hit
CGTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGC7170.18767275316190635No Hit
GTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCG7130.18662576430186784No Hit
CTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGT7070.18505528101181004No Hit
GGTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTC6710.17563238127146327No Hit
CCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGGCGGTC6640.17380015076639585No Hit
GCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGCGAAAGCAGGTCA6540.17118267861629952No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC6170.16149803166094312No Hit
GCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCC6150.16097453723092386No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA6120.16018929558589495No Hit
CCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTTT6110.15992754837088535No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTG6080.15914230672585644No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA5830.15259862635061563No Hit
GGCCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTG5820.15233687913560598No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTG5710.14945765977050005No Hit
GCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC5690.1489341653404808No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG5670.1484106709104615No Hit
CCAGCCTACACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCT5580.14605494597537483No Hit
GCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGC5500.14396096825529775No Hit
CGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTC5470.14317572661026887No Hit
CCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGAGAACCA5450.1426522321802496No Hit
CAAACTGCGAATACCGGAGAATGTTATCACGGGAGACACACGGCGGGTGC5430.14212873775023033No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG5400.14134349610520144No Hit
GTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTC5300.13872602395510514No Hit
TCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCG5260.13767903509506657No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC5220.13663204623502806No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT5120.13401457408493173No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA5100.13349107965491247No Hit
GTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGA5030.13165884914984505No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTA5000.13087360750481614No Hit
CCGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGT5000.13087360750481614No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG4990.13061186028980654No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG4970.13008836585978725No Hit
GAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGT4940.12930312421475834No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA4920.12877962978473909No Hit
GCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG4920.12877962978473909No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC4890.1279943881397102No Hit
CCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCC4810.12590041041963315No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGT4720.12354468548454645No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4640.1214507077644694No Hit
CGGAGAATGTTATCACGGGAGACACACGGCGGGTGCTAACGTCCGTCGTG4590.12014197168942121No Hit
GTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTTTCT4560.11935673004439232No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC4540.11883323561437306No Hit
CGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCC4530.11857148839936343No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA4530.11857148839936343No Hit
CCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACC4520.11830974118435379No Hit
GAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTAT4500.11778624675433454No Hit
CACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGG4470.11700100510930564No Hit
CCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCT4450.11647751067928637No Hit
GCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT4430.11595401624926711No Hit
GGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAA4370.11438353295920932No Hit
GGGAGACACACGGCGGGTGCTAACGTCCGTCGTGAAGAGGGAAACAACCC4350.11386003852919004No Hit
GGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGG4330.1133365440991708No Hit
GCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTA4310.11281304966915151No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG4310.11281304966915151No Hit
CTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGT4220.11045732473406483No Hit
GCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCG4220.11045732473406483No Hit
GCCGCAGCTTCGGTGCATGGTTTAGCCCCGTTACATCTTCCGCGCAGGCC4200.10993383030404556No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG4200.10993383030404556No Hit
CGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATCACTATGACCTGC4180.10941033587402631No Hit
GTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCC4170.10914858865901667No Hit
GCAATGGCATAAGCCAGCTTGACTGCGAGCGTGACGGCGCGAGCAGGTGC4170.10914858865901667No Hit
GGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGG4150.10862509422899741No Hit
CTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGGCG4120.10783985258396851No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT4120.10783985258396851No Hit
CCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCG4070.10653111650892033No Hit
TCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCAT4020.10522238043387218No Hit
CTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGA4010.10496063321886256No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3980.10417539157383365No Hit
CAGTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTT3900.1020814138537566No Hit
TCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCAT3880.10155791942373733No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT3850.10077267777870844No Hit
TCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGG3850.10077267777870844No Hit
CCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGGGGAGAACC3830.10024918334868918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC2100.0110.476199
ATTGGGA1650.0101.060612
GATTGGG1850.094.054061
GAGAGGG4300.087.674427
ATCGGGA2950.081.101692
GGAGAGG4950.077.626266
GCAGGGG2400.075.520836145
GATCGGG3350.069.253731
GCCCGGA955.456968E-1268.6842041
TTGGGAG2250.067.666673
CGTCGCT2900.065.000011
GGGAGAG6900.064.094215
GAGTACC7000.055.9285746
CGGAGTA6750.055.851854
CGGAGAG1301.8189894E-1255.7692345
AGTACCT7200.054.3749967
GGAGTAC7250.054.05
CGGGCAC553.2164803E-452.7272721
GAGAGCG2350.052.4468087
AGAGGGG7450.051.5771878
Produced by FastQC (version 0.11.2)