FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_64n2.3520000009e8bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_64n2.3520000009e8bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433518
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA32880.7584460160823772No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG31810.7337642266295747No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA25500.5882108701368801No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC24360.5619143841778196No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA23310.5376939365839482No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA22020.5079373866829059No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT18400.4244345102164155No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA18350.42328115556908824No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG17140.39536997310376965No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG16760.38660447778408275No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC16510.3808377045474467No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC15390.3550025604473171No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT15160.34969712906961187No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA15010.3462370651276302No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG14500.3344728477248926No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA14140.32616869426413664No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT13910.32086326288643147No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG13870.31994057916856966No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC13010.3001028792345416No Hit
GCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT12630.29133738391485475No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC10950.25258466776466026No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGCT10850.25027795847000583No Hit
CCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCA10660.24589521081016244No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG10230.2359763608431484No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG10200.23528434805475204No Hit
TCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGG9680.223289459722549No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT9660.22282811786361814No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT9570.22075207949842912No Hit
CTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCAG9350.21567731905018936No Hit
GCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAG9310.2147546353323276No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT9170.2115252423198114No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT9150.21106390046088053No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG9090.20967987488408785No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG9080.20944920395462244No Hit
CACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACC9030.2082958493072952No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG8820.20345175978852087No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT8710.200914379564401No Hit
GTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTT8530.19676230283402302No Hit
CGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCT8440.19468626446883405No Hit
CCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGC8350.19261022610364506No Hit
GGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGT8280.19099552959738697No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC8280.19099552959738697No Hit
CTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTC8140.18776613658487076No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC8050.18569009821968177No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC7900.18223003427770013No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG7830.180615337771442No Hit
GGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCT7800.17992332498304567No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA7790.17969265405358023No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAG7760.17900064126518392No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA7750.17876997033571848No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA7460.17208051338122063No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT7330.16908179129816986No Hit
CTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAG7200.16608306921511912No Hit
CTACACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCC7030.16216166341420657No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACA6770.15616421924810503No Hit
ATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTG6640.15316549716505426No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTG6610.15247348437665795No Hit
TCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA6590.15201214251772707No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT6470.14924409136414174No Hit
GTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAA6370.1469373820694873No Hit
CTTGCCCCGAGATGAGTTCTCCCTGACTCCTTGAGGGTCCTGAAGGAACG6370.1469373820694873No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC6320.1457840274221601No Hit
CTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACC6300.14532268556322922No Hit
GTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACGGAA6210.1432466471980402No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA6110.1409399379033858No Hit
GGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGG5960.13747987396140415No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA5960.13747987396140415No Hit
TAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGA5940.13701853210247325No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTG5920.13655719024354235No Hit
CGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGG5910.1363265193140769No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT5890.13586517745514604No Hit
CACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCCGTC5880.1356345065256806No Hit
CACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAG5670.1307904170069063No Hit
CACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGG5560.1282530367827864No Hit
GTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGA5560.1282530367827864No Hit
GCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTA5550.12802236585332097No Hit
GTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACATAT5420.1250236437702702No Hit
CCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGG5380.12410096005240843No Hit
CCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCG5320.12271693447561578No Hit
CAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGC5240.12087156703989223No Hit
GGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCA5200.11994888332203046No Hit
CCGAGATGAGTTCTCCCTGACTCCTTGAGGGTCCTGAAGGAACGTTGAAG5140.11856485774523778No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA5090.11741150309791058No Hit
GTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTT5060.11671949030951426No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA5050.1164888193800488No Hit
CTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTAC5040.11625814845058337No Hit
CTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACATATCAGC4900.11302875543806716No Hit
GTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCG4890.11279808450860172No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4870.11233674264967083No Hit
CAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATG4760.10979936242555095No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC4760.10979936242555095No Hit
GCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGA4760.10979936242555095No Hit
ATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCC4680.10795399498982743No Hit
GGCAGGCGTCACACCGTATACGTCCACTTTCGTGTTTGCACAGTGCTGTG4640.10703131127196563No Hit
CTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTC4610.10633929848356931No Hit
GTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTG4580.10564728569517298No Hit
GCAGCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTT4570.10541661476570754No Hit
TGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCA4560.10518594383624208No Hit
GCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCC4530.10449393104784575No Hit
CCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA4450.10264856361212223No Hit
GTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTAC4350.10034185431746778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC4750.0100.736849
AGAGCGG2850.086.491238
ATCGGGA3850.084.7402652
ATTGGGA1400.082.857142
GAGAGCG3550.079.647897
AACGGGA2300.078.804352
GAGAGGG7500.078.37
GGAGAGG7850.077.579616
GATCGGG4400.075.7954561
GAGCGGC2850.071.228079
GGAGAGC4450.063.539326
GGGAGAG13250.062.377365
AAGGGGC1101.6716513E-952.7272769
GATTGGG2350.052.4468081
ACGGGAG3550.051.056343
GCAGGGG4050.050.123455145
CCCCGGT4000.048.9374961
GAGCGGT901.321825E-648.3333329
GGTTCGC4950.046.8686875
AGAGGGG13300.046.3345878
Produced by FastQC (version 0.11.2)