FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_64n1.3520000009e8a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_64n1.3520000009e8a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358917
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG29640.8258176681516898No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA29000.8079862475168355No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC25600.7132568253941719No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA23810.6633845708060638No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA21980.6123978524282773No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA21510.5993029028995561No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG19330.5385646263620837No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT18850.525191060885943No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC17230.4800552774039681No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG16580.46194524082169414No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT15240.42461070386746796No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA14810.41263021812842526No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG14080.39229125396679454No Hit
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC13570.378081840648395No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC13550.37752460875355587No Hit
GTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCC12430.34631962264256083No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGCT12290.3424189993786864No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG11780.3282095860602869No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC11090.3089850856883346No Hit
CCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTCACCGACGC10790.3006266072657467No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT10010.278894563367018No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT9800.27304362847120645No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA9740.27137193278668886No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT9580.26691407762797525No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG9540.26579961383829687No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT9450.26329207031152047No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG9300.2591128311002265No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT9190.25604805567861094No Hit
CCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAG9080.25298328025699535No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG8660.2412814104653722No Hit
GCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT8170.22762922904181188No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG7870.21927075061922396No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC7760.21620597519760834No Hit
GTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGG7630.21258396788115358No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC7510.20924057651211841No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT7470.20812611272244No Hit
GCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAG7200.20060348214211085No Hit
CCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCA7150.19921040240501284No Hit
CTACACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCC7100.19781732266791488No Hit
TAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGA6970.1941953153514601No Hit
CGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCT6900.19224500371952288No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA6790.18918022829790732No Hit
GGTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTC6780.18890161235048772No Hit
CGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGG6770.18862299640306812No Hit
GGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCT6720.18722991666597014No Hit
GGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGT6710.1869513007185505No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC6580.18332929340209575No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG6380.17775697445370378No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC6310.1758066628217666No Hit
CTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTC6210.1730205033475706No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC6170.17190603955789222Illumina Single End PCR Primer 1 (97% over 35bp)
TCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA6090.16967711197853544No Hit
CTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCAG6070.16911988008369624No Hit
GTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAA6060.16884126413627662No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA6010.16744818439917863No Hit
GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTG5980.16661233655691984No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT5730.1596469378714299No Hit
CACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGG5670.1579752421869123No Hit
CCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCG5590.1557463146075555No Hit
GGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGG5580.1554676986601359No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA5440.15156707539626152No Hit
GTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTT5410.15073122755400276No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT5380.14989537971174394No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA5340.14878091592206555No Hit
CTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACC5290.14738783618496756No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA5130.14292998102625398No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5130.14292998102625398No Hit
CTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGA5070.14125828534173637No Hit
CACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACC4990.1390293577623796No Hit
GGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTGA4900.13652181423560322No Hit
GTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTA4820.13429288665624645No Hit
CACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCCGTC4810.13401427070882682No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT4770.13289980691914843No Hit
GTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGA4700.13094949528721123No Hit
AGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAG4670.13011364744495246No Hit
GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG4670.13011364744495246No Hit
GCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTA4610.12844195176043488No Hit
CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT4560.12704887202333687No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4550.12677025607591727No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA4530.12621302418107808No Hit
GACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCG4510.12565579228623888No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACA4420.1231482487594625No Hit
GTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGGCAAGACGGAA4290.11952624144300772No Hit
AAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGGAGC4200.11701869791623132No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC4190.11674008196881173No Hit
GTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTG4140.11534700223171375No Hit
CTCGCCTTAGGGGTCGACTCACCCTGCCCCGATTAACGTTGGACAGGAAC4100.11423253844203535No Hit
GTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGC4090.11395392249461574No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4050.11283945870493735No Hit
ATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCC4020.11200361086267856No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC3990.11116776302041975No Hit
GCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCC3940.10977468328332177No Hit
AAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGA3830.10670990786170619No Hit
CTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTAC3750.1044809802823494No Hit
CGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAG3720.10364513244009059No Hit
CCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGGCTG3650.10169482080815341No Hit
CTTGCCCCGAGATGAGTTCTCCCTGACTCCTTGAGGGTCCTGAAGGAACG3620.10085897296589462No Hit
CCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCC3620.10085897296589462No Hit
GTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTT3610.10058035701847504No Hit
CCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGCGG3600.10030174107105543No Hit
CCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTACGGGGCT3590.10002312512363581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGGA5950.0136.47062
GAGGGGC9650.0129.222819
ATCGGGA8700.0125.000012
TTGGGAG6800.0120.4779363
GATTGGG7550.0113.311271
GATCGGG10550.0109.952611
GAGAGCG6700.0107.126867
AGAGCGG6650.0103.571428
GGAGAGC7000.0102.535716
GAGCGGC5200.0101.7788549
GGAGAGG16500.097.9848566
AACGGGA2600.097.596162
GAGAGGG16200.097.11427
CGGAGAG2350.089.468095
GGGAGAG23950.086.8789145
AAGGGGC2100.082.857159
GATCCGA353.4342687E-582.857141
TCGGGAG13600.075.1654363
TCCGGAG1650.074.696963
AAGCGGC1150.069.347839
Produced by FastQC (version 0.11.2)