FastQCFastQC Report
Thu 28 Sep 2017
000000000-BD6BC_l01n02_63n2.3520000009e879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BD6BC_l01n02_63n2.3520000009e879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362781
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC46591.2842458673414539No Hit
GGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGG33620.9267299004082352No Hit
GGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTA27250.7511418734718742No Hit
GCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCA27170.7489366863203972No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA26230.7230257372905416No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG25050.690499226806255No Hit
GGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGT23410.6452928902009752No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA21400.5898875630201141No Hit
CTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACCAG20010.5515724362632002No Hit
GTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGA17240.47521783114330685No Hit
CTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGT15380.42394722987146516No Hit
GGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAG13470.3712983866299503No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACA11610.3200277853581086No Hit
CCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCC9770.26930848087413617No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC9100.2508400384805158Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAAC8110.22355084748098714No Hit
GTGACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC7970.2196917699659023No Hit
CTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCG7820.21555704405688278No Hit
GGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAG7790.2147300988750789No Hit
GAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTAT7610.20976842778425553No Hit
GCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGG7440.20508240508736675No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCT7300.20122332757228192No Hit
CAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATCAAAT7070.19488341451178534No Hit
CGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGT6940.19129998539063514No Hit
CTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG6650.18330618196653078No Hit
TGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGT6390.17613932372423033No Hit
CTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACATATCAGC6030.16621598154258355No Hit
TCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCAT5990.16511338796684502No Hit
CGGCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTC5920.1631838492093026No Hit
GCCTCGTCATCACGCCTCAGCCTTGGTTTTCCGGATTTGCCTGGAAAACC5820.16042736526995627No Hit
CCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCA5630.15519004578519824No Hit
ATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCC5580.15381180381552506No Hit
CTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAG5570.15353615542159044No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC5560.1532605070276558No Hit
CAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGA5550.1529848586337212No Hit
GTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTG5450.15022837469437486No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA5440.1499527263004402No Hit
CCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCA5430.14967707790650558No Hit
GGCCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCT5410.1491257811186363No Hit
GCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACA5380.1482988359368324No Hit
CTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGG5360.14774753914896316No Hit
GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC5290.1458180003914207No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC5240.14443975842174755No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC5180.14278586805813975No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA5130.14140762608846658No Hit
GGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGT5050.13920243893698955No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGA4880.13451641624010077No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGAGCTATTACGCTT4860.13396511945223152No Hit
GGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCT4720.13010604193714664No Hit
CCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT4720.13010604193714664No Hit
CTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACC4610.1270739096038657No Hit
GTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGC4600.12679826120993107No Hit
TGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTG4540.12514437084632327No Hit
CCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT4520.124593074058454No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG4480.12349048048271545No Hit
TGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTG4470.12321483208878083No Hit
TCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGC4440.12238788690697694No Hit
CGTCGCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCA4390.12100964493730379No Hit
CCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGA4350.11990705136156524No Hit
CAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCG4320.11908010617976135No Hit
GCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTC4290.11825316099795743No Hit
CCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTGTCG4210.11604797384648038No Hit
GTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGA4210.11604797384648038No Hit
CCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA4140.11411843508893796No Hit
GCGACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCATCAGGTAACATC4130.11384278669500332No Hit
CACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAA4060.1119132479374609No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG4010.11053500596778774No Hit
GTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTT4000.11025935757385309No Hit
CTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACC3950.10888111560417993No Hit
ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG3880.1069515768466375No Hit
TCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA3800.10474638969516044No Hit
GTGGCCTGTGAGGGTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAA3750.10336814772548729No Hit
GTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGG3730.10281685093761803No Hit
CGTTCTGTAAGCCTGTGAAGGTGGCCTGTGAGGGTTGCTGGAGGTATCAG3710.10226555414974874No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA3650.10061166378614096No Hit
TGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAA3640.10033601539220632No Hit
CTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGT3630.10006036699827167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA16300.0137.438642
GAGAGCG11650.0128.819757
GATCGGG17950.0127.632321
GAGCGGC10300.0127.4029169
GGAGAGG26000.0127.1538546
GAGGGGC21400.0124.3341149
AGAGCGG12050.0123.340258
GGAGAGC12650.0122.648226
GAGAGGG26900.0121.821577
GGGAGAG37150.0118.849265
ATTGGGA9000.0118.416662
AACGGGA6750.0118.148152
AAGCGGC2400.0117.81259
AAGGGGC6150.0116.707329
GATTGGG9500.0115.236851
TCGGGAA5050.0104.801993
CGGGAAA5950.0104.789924
GTCGGGA3150.0103.571432
CGGAGAG4100.097.2560965
ACGGAAG2200.095.568183
Produced by FastQC (version 0.11.2)